69 Best Ai Startups in Czech Republic to Watch in 2026

The Definitive Seedtable Ranking of Ai Startups in Czech Republic

We track 71,000+ companies and rank them dynamically using our Seedtable Score – a score that uses quantitative and qualitative data points to signal the momentum behind a company. We then monitor the list manually leveraging our expertise as founders and investors.

There are 394 start-ups with an aggregate funding of $21.4b. The average funding per company in this subset is $309.9m.

Last update to the database: May 19, 2026. See changelog.

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6

Funding Rounds

$1.5b

Money raised

SambaNova Systems is a startup that focuses on machine learning and big data analytics, founded by Kunle Olukotun and Rodrigo Liang.

7

Funding Rounds

$280.9m

Money raised

AppZen is a San Jose, California-based company that provides an AI-based auditing platform for finance teams.

Key people:

4

Funding Rounds

$325.0m

Money raised

Synchron, Inc. is a technology company building implantable neural interface solutions, including endovascular brain-computer interface.

7

Funding Rounds

$350.3m

Money raised

Snorkel AI is a data-centric artificial intelligence company born out of Stanford University's AI Lab.

3

Funding Rounds

$764.7m

Money raised

Bitmain is a Beijing-based multinational semiconductor company, founded in 2013, that manufactures products for blockchain and artificial intelligence (AI) applications.

10

Funding Rounds

$1.0b

Money raised

DataRobot sells a machine learning platform that helps users build and deploy accurate predictive models. The Boston-based company was founded in 2012 by Jeremy Achin.

5

Funding Rounds

$390.0m

Money raised

PathAI is a Boston-based artificial intelligence company developing therapeutics and healthcare products.

10

Funding Rounds

$930.0m

Money raised

Tempus, a technology company advancing precision medicine through the practical application of artificial intelligence in healthcare.

7

Funding Rounds

$617.0m

Money raised

Lemonade is an insurance technology company using artificial intelligence and behavioral economics to offer home and renters insurance.

6

Funding Rounds

$317.0m

Money raised

BigPanda offers a platform that helps IT Ops, NOC, and DevOps teams detect, investigate, and resolve IT incidents.

6

Funding Rounds

$80.5m

Money raised

Hologram is a company with a platform for building Internet of Things type products

Key people:

5

Funding Rounds

$75.9m

Money raised

Unstoppable Domains is a company that offers alt-route domain names based on blockchain technology.

7

Funding Rounds

$321.1m

Money raised

Deep Instinct is a Tel Aviv- and New York City-based cybersecurity company that applies deep learning to cybersecurity.

Key people:

6

Funding Rounds

$159.5m

Money raised

Evernote is a company offering notetaking software and online service founded in 2008 by Stepan Pachikov.

7

Funding Rounds

$1.5b

Money raised

Dataminr is a company providing a real-time AI platform delivering information on high-impact events and breaking news.

Key people:

5

Funding Rounds

$235.0m

Money raised

Udacity is an e-learning company that has a digital education platform. It was founded in 2011 and is located in Mountain View, California.

4

Funding Rounds

$153.5m

Money raised

Sherlock Biosciences is a Cambridge, Massachusetts-based company founded in 2019 by David R. Walt, Rahul Dhanda, Feng Zhang, Jim Collins, Deborah Hung, Jonathan Gootenberg, Omar Abudayyeh, Pardis Sabeti and Todd Golub.

8

Funding Rounds

$967.6m

Money raised

Freenome is a San Francisco-based company which uses genetic analysis for early cancer detection and risk assessment.

3

Funding Rounds

$305.0m

Money raised

Moveworks is an AI, machine learning, enterprise natural language understanding (NLU) and chatbot platform that resolves IT Service Desk support issues.

6

Funding Rounds

$399.8m

Money raised

Human Longevity is a genomic-based, health intelligence company empowering proactive healthcare and enabling a life better lived.

Key people:

6

Funding Rounds

$96.3m

Money raised

Anomali is a Redwood City, California-based cybersecurity company offering real-time threat intelligence capabilities.

6

Funding Rounds

$214.0m

Money raised

Observe.AI is the creator of an AI platform for call centers.

8

Funding Rounds

$286.6m

Money raised

SparkCognition is an artificial intelligence and cybersecurity company offering cognitive computing analytics for the information technology, operational technology, and the internet of things (IoT) industries.

2

Funding Rounds

$98.0m

Money raised

OrphAI Therapeutics is a biotechnology research company based in Guilford, Connecticut that develops precision therapeutics technology designed to treat cancer and rare diseases.

4

Funding Rounds

$58.0m

Money raised

3T Biosciences is a biotechnology company developing immunotherapies.

Key people:

1

Funding Rounds

$50.0m

Money raised

VALR is a company founded in 2018 by Farzam Ehsani, theo bohnen and badi sudhakaran.

10

Funding Rounds

$588.1m

Money raised

Shield AI is a company building artificially intelligent systems like drones for use by military service members and first responders.

2

Funding Rounds

$122.5m

Money raised

Together is a San Francisco-based developer of a decentralized cloud for artificial intelligence.

3

Funding Rounds

$34.7m

Money raised

Gideon is a robotics company that creates products for industrial settings.

4

Funding Rounds

$45.0m

Money raised

Company providing service to identify and classify textual content with Artificial Intelligence

7

Funding Rounds

$30.3m

Money raised

Mattermark is a San Francisco-based company founded by Kevin Morrill, Danielle Morrill and Andy Sparks.

Key people:

2

Funding Rounds

$23.6m

Money raised

Molecula is a data virtualization company that enables instantaneous, secure access to large, fragmented and geographically dispersed datasets to support demanding Machine Learning (ML) and Artificial Intelligence (AI) workloads.

Key people:

7

Funding Rounds

$249.0m

Money raised

Eko Health is a digital health and AI technology company developing tools for heart and lung disease detection.

Key people:

5

Funding Rounds

$200.0m

Money raised

People.ai is an AI platform based on sales and marketing.

3

Funding Rounds

$17.5m

Money raised

Numerai is a hedge fund with stock market trades determined by crowdsourced artificial intelligence models. The best models receive rewards in the form of a cryptographic token called Numeraire (NMR) on the Ethereum blockchain.

8

Funding Rounds

$390.0m

Money raised

Exabeam is a San Mateo, California-based data security analytics company offering cybersecurity and early cyber attack detection software.

8

Funding Rounds

$646.9m

Money raised

Socure is a New York City-based developer of an identity verification and fraud risk solution.

8

Funding Rounds

$71.3m

Money raised

Notable Labs is a company providing personalized drug combination testing service for cancer patients.

7

Funding Rounds

$169.8m

Money raised

An artificial intelligence company offering a call tracking and conversational analytics for marketers.

4

Funding Rounds

$138.9m

Money raised

Verkada is a software company offering a cloud-based internet of things (IoT) platform for security camera systems.

Key people:

6

Funding Rounds

$103.4m

Money raised

Tribal Credit is a company in San Francisco.

9

Funding Rounds

$1.2b

Money raised

A company developing artificial intelligence-based technology designed for autonomous vehicles. It was founded in 2016 and is located in Fremont, California.

8

Funding Rounds

$3.5b

Money raised

SentinelOne is a U.S-based company providing endpoint security software globally founded in 2013 by Tomer Weingarten, Ehud Shamir and Almog Cohen.

6

Funding Rounds

$107.6m

Money raised

Zapata Computing is a company developing quantum software and algorithms for business founded in 2017 by Christopher Savoie, Alán Aspuru-Guzik, Jhonathan Romero Fontalvo, Jonathan Olson, Peter Johnson and Yudong Cao.

Key people:

4

Funding Rounds

$73.0m

Money raised

Wickr is a telecommunications and security company that builds secure communication tools, such as a messaging application. It was founded in 2012 and is located in San Francisco, California.

Key people:

15

Funding Rounds

$249.8m

Money raised

Sift Science is a San Francisco-based company founded in 2011 by Brandon Ballinger.

12

Funding Rounds

$296.1m

Money raised

BigID is a cybersecurity company developing a platform for data protection and privacy compliance.

Key people:

4

Funding Rounds

$743.2m

Money raised

A bioinformatics company in San Francisco building data collection methods and databases that will enable machine learning algorithms in healthcare. It was founded in 2018.

5

Funding Rounds

$62.7m

Money raised

QC Ware Corp. is a company that offers quantum computing software solutions for enterprises.

Key people:

6

Funding Rounds

$525.0m

Money raised

Forter is a E-Commerce fraud detection platform founded in 2013 by Michael Reitblat, Liron Damri and Alon Shemesh.

Key people:

2

Funding Rounds

$37.0m

Money raised

Blue Hexagon is a San Francisco-based cybersecurity company offering an on-device machine learning based malware detection product.

Key people:

5

Funding Rounds

$42.5m

Money raised

Textio is a writing-enhancement service powered by big data.

Key people:

6

Funding Rounds

$15.8m

Money raised

Massive Bio is a New York-based company founded by Selin Kurnaz, Dr. Arturo Loaiza-Bonilla and Cagatay M. Culcuoglu, offering precision oncology services to cancer patients, providers, and pharmaceutical companies.

3

Funding Rounds

$31.2m

Money raised

Deep Lens is an Artificial Intelligence company matching patients with clinical trials.

Industries:

Key people:

3

Funding Rounds

$47.0m

Money raised

Spatial has created a metaverse designed to give brands and creators a virtual space to display their digital works.

4

Funding Rounds

$20.1m

Money raised

Call analytics platform that uses artificial intelligence to transcribe and analyze sales calls, providing enterprises with actionable feedback to improve the performance of their sales teams.

3

Funding Rounds

$38.2m

Money raised

Tact.ai is an A.I. assistant company founded in 2012 by Chuck Ganapathi.

3

Funding Rounds

$14.3m

Money raised

Aria Pharmaceuticals is a first-in-class pharmaceutical discovery and development founded in 2014 by Andrew M. Radin.

4

Funding Rounds

$3.2m

Money raised

MonkeyLearn is an artificial intelligence data analysis and visualization platform.

6

Funding Rounds

$9.8m

Money raised

Facial recognition company Kairos today announced the alpha release of the Kairos Protocol, a biometric verification system for cryptocurrency transactions.

4

Funding Rounds

$7.0m

Money raised

An artificial intelligence company developing a platform that helps analysts process and understand information.

2

Funding Rounds

$15.5m

Money raised

Moesif is a Caifornia-based company providing API analytics and tools.

3

Funding Rounds

$15.0m

Money raised

Biotechnology company developing proteins from algae for animal and human health and address world's food shortages

Key people:

3

Funding Rounds

$2.3m

Money raised

A web and API platform that provides Bitcoin and Altcoin conversion.

3

Funding Rounds

$2.9m

Money raised

Artificial Intelligence company using Augmented Reality for collaborative professional services

Key people:

12

Funding Rounds

$2.8m

Money raised

HelixNano is a Cambridge, Massachusetts-based biotechnology and artificial intelligence company founded in 2013 by Hannu Rajaniemi and Nikolai Eroshenko.

1

Funding Rounds

$510.0k

Money raised

Wavel.ai is a voice AI solution for scaling and localizing video content.

2

Funding Rounds

Hypergiant Industries is a machine learning and artificial intelligence company offering client-focused services and research and development in various fields. The company was founded by Ben Lamm and John Fremont in 2018.

3

Funding Rounds

$54.1k

Money raised

ApexQubit is a biotechnology company that is harnessing a proprietary technology for discovery of new medicines to treat patients with undruggable diseases.

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